Journal: International Journal of Medical Sciences
Article Title: Hyperbaric oxygen protects against periodontal bone loss by modulating inflammation and bone remodeling via RANKL/OPG expression in ligature-induced periodontitis
doi: 10.7150/ijms.122857
Figure Lengend Snippet: HBOT reduces gingival cytokine and chemokine expression in ligature-induced periodontitis. (A) Representative cytokine array blots showing expression profiles of 10 key inflammatory mediators (red boxes) in gingival tissues collected at day 14 and day 28 from Sham, PD+RECOV (natural recovery), PD (periodontitis only), PD+EHBOT (early HBOT), and PD+LHBOT (late HBOT) groups. The targeted proteins included: 1. CINC-1 (CXCL1), 2. CINC-2α/β (CXCL3), 3. sICAM-1, 4. IL-1α, 5. IL-1β, 6. IL-1 receptor antagonist (IL-1ra), 7. LIX (CXCL5), 8. L-selectin (CD62L), 9. Thymus chemokine (CCL25), and 10. TIMP-1. (B) Quantitative analysis of cytokine and chemokine expression at day 14. Periodontitis induced marked increases in several pro-inflammatory cytokines and chemokines. Early HBOT significantly suppressed most inflammatory mediators compared to the PD and PD+RECOV groups. (C) Quantitative analysis at day 28. PD-induced cytokine elevation persisted, while both early and late HBOT treatments effectively reduced IL-1α, IL-1β, IL-1ra, sICAM-1, CINC-1, CINC-2α/β, LIX, and thymus chemokine levels. Notably, early HBOT more effectively reduced thymus chemokine expression than natural recovery. Data are presented as mean ± SD. n = 6 per group. Panel 2A shows a cytokine dot-array membrane (R&D Systems ARY008) from a single, intact exposure; the published panel was border-cropped only to remove blank margins (global linear contrast, no compositing). See Supplementary for the full, uncropped membrane and original X-film/RAW.
Article Snippet: Protein extracts (200 μg per sample) were applied to Rat Cytokine Antibody Arrays (ARY008, R&D Systems, Inc., USA), which detect a panel of 34 inflammatory cytokines, chemokines, and adhesion molecules.
Techniques: Expressing, Membrane
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